ABSTRACT
The rhizome part of Cyperus rotundus has been shown to contain 5-hydroxymethyl furfural (HMF) (1), methyl ferulate (MF) (2), (E)-ferulaldehyde (FA) (3), and N-trans-feruloyl tyramine (NTF) (4).These known compounds are being reported for the first time from this plant and their structures were determined by physical properties and spectroscopic analyses. The present study was designed to investigate the analgesic and anti-inflammatory activities of methanol, chloroform and ethyl acetate extracts of C. rotundus. All the extract displayed significant analgesic effect in acetic acid and hot plate pain models in a dose dependent manner. As compared with the other extracts, the ethyl acetate extract (500 mg/kg) was the most effective in the analgesic test similar to standard drugs. In writhing test, ethyl acetate extract (500 mg/kg) showed significantly, inhibiting pain by 73.44 %, similar to aspirin which showed 76.47 % inhibition at the dose of 100 mg/kg i.p. In hot plate test, ethyl acetate extract (500 mg/kg) produced maximum possible analgesia of 12.72 ± 1.15 sec at 90 min, whilst morphine sulfate (5 mg/kg) showed 13.83 ± 1.15 sec. Similarly, carrageenan-induced paw volume was significantly reduced by ethyl acetate extract (500 mg/kg) at 2.20 ± 1.18 4 h after administration similar to that of diclofenac sodium which showed 2.24 ± 1.18 at the dose of 100 mg/kg. The data justify the traditional use of Cyperus rotundus as medicinal plant which has a potential source of bioactive molecules to treat inflammatory diseases.
ABSTRACT
Clitoria ternatea belonging to the family Fabaceae, commonly known as Butterfly pea. A wide range of secondary metabolites including triterpenoids, flavonol glycosides, anthocyanins and steroids were isolated from Clitoria ternatea Linn. GC-MS analyses of CHCl3 and EtOAc fractions were performed on Gas Chromatography with mass spectrometer. The aim of the present study was to evaluate the radical scavenging ability, using the stable radical DPPH and anti-diabetic activity in alloxan induced diabetes in albino rats was evaluated from the flowers extract of Clitoria ternatea. The blood glucose level in the different groups was assayed and biochemical parameters were assessed to support the proposed hypothesis. During the experimental period, body weight of all the rats was determined at regular intervals of time. The serum glucose lowering activity was compared with glibenclamide, a standard hypoglycemic drug.
ABSTRACT
Leucas aspera (Willd.) Linn. (Lamiaceae), commonly known as “Thumbai,” is distributed throughout India. This study includes the isolation and characterization of flavonoids present in Leucas aspera flowers were compared wound healing activity with standard soframycin ointment. The plant materials were extracted with 95% methanol, petroleum ether, ethyl acetate, and were subjected to column and TLC chromatographic separation analysis. The chemical constituents isolated from the flowers of L. aspera were characterized based on chemical tests and spectral analysis such as UV and NMR spectroscopy. The structure of the isolated compound was confirmed as baicalein-7-O- β-D-glucuronide (baicalin). It has been investigated for wound healing activity by applying on the albino rats. A slow rate of healing was observed earlier which turns to very rapid on the 12th day. The histopathological examination provided additional evidence for the experimental wound healing studies. Protease is a biochemical marker and pH measured to support the wound healing activity. We conclude that the baicalin isolated with the flowers of L. aspera has better wound healing activity.